abstract：:In this work, we explore the use of the unbiased cDNA-AFLP strategy to identify genes involved in Mn(2+) homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been sequenced, three Mn peroxidase genes responding to Mn(2+) have been characterized. Using cDNA-AFLP to...

journal_title：Current genetics

authors： Gutiérrez M,Rojas LA,Mancilla-Villalobos R,Seelenfreund D,Vicuña R,Lobos S

更新日期：2008-10-01 00:00:00

abstract：:The unicellular green alga Chlamydomonas reinhardtii has emerged as a superb model species in plant biology. Although the alga is easily transformable, the low efficiency of transgene expression from the Chlamydomonas nuclear genome has severely hampered functional genomics research. For example, poor transgene expres...

journal_title：Current genetics

authors： Shao N,Bock R

更新日期：2008-06-01 00:00:00

abstract：:Microbial pathogens secrete an array of cell wall-degrading enzymes to break down the structure of the host cell wall to facilitate colonization of the host tissue. To better understand their role in the pathogenesis, a putative endoglucanase from Magnaporthe grisea was characterized in this paper. SignalP-3.0 analysi...

journal_title：Current genetics

authors： Zhou J,Zheng XZ,Lan L,Lin CZ,Wu YB,Lin XJ,Ebbole D,Lu GD,Wang ZH

更新日期：2008-04-01 00:00:00

abstract：:Retrotransposons are suitable targets for developing molecular markers for population genetics studies. Transposable elements have not yet been isolated from ectomycorrhizal fungi of the genus Tuber. In this paper, we report on the isolation and characterization of Tmt1, an LTR-retrotransposon from Tuber melanosporum....

journal_title：Current genetics

authors： Riccioni C,Rubini A,Belfiori B,Passeri V,Paolocci F,Arcioni S

更新日期：2008-01-01 00:00:00

abstract：:Cell fusion occurs regularly during the vegetative and sexual phases of the life cycle in filamentous fungi. Here, we present a simple and efficient method that can detect even rare hyphal fusion events. Using the homothallic ascomycete Sordaria macrospora as an experimental system, we developed a histone-assisted mer...

journal_title：Current genetics

authors： Rech C,Engh I,Kück U

更新日期：2007-11-01 00:00:00

abstract：:The separation and identification of siderophores produced by microorganisms is a time-consuming and an expensive procedure. We have developed a new and efficient method to identify siderophores using well-established Saccharomyces cerevisiae deletion mutants. The Deltafet3,arn strains fail to sustain growth, even whe...

journal_title：Current genetics

authors： Park YS,Kim JH,Chang HI,Kim SW,Paik HD,Kang CW,Kim TH,Sung HC,Yun CW

更新日期：2007-09-01 00:00:00

abstract：:A main function of ectomycorrhizas, a symbiosis between certain soil fungi and fine roots of woody plants, is the exchange of plant-derived carbohydrates for fungus-derived nutrients. As it is required in large amounts, nitrogen is of special interest. A gene (AmAMT2) coding for a putative fungal ammonium importer was...

journal_title：Current genetics

authors： Willmann A,Weiss M,Nehls U

更新日期：2007-02-01 00:00:00

abstract：:Glycerophosphoinositol (GroPIns) and glycerophosphocholine (GroPCho) are the products of phospholipase-B mediated deacylation of phosphatidylinositol and phosphatidylcholine, respectively. GroPIns and GroPCho are transported across the Saccharomyces cerevisiae plasma membrane into the cell via the transporter encoded ...

journal_title：Current genetics

authors： Almaguer C,Fisher E,Patton-Vogt J

更新日期：2006-12-01 00:00:00

abstract：:Spindle pole body (SPB; the microtubule organizing center in yeast) duplication is essential to form a bipolar spindle. The duplicated SPBs must then separate and migrate to opposite sides of the nucleus. We identified a novel functional relationship in SPB separation between the microtubule stabilizing protein Bik1p/...

journal_title：Current genetics

authors： Strawn LA,True HL

更新日期：2006-12-01 00:00:00

abstract：:The isoprenoid pathway of the ectomycorrhizal fungus Tuber borchii Vittad is investigated to better understand the molecular mechanisms at work, in particular during the maturation of the complex ascomata (the so-called "truffles"). Three T. borchii genes coding for the most important regulatory enzymes of the isopren...

journal_title：Current genetics

authors： Guidi C,Zeppa S,Annibalini G,Pierleoni R,Guescini M,Buffalini M,Zambonelli A,Stocchi V

更新日期：2006-12-01 00:00:00

abstract：:Laccases are phenol-oxidizing, multicopper enzymes produced by fungi, plants, insects and bacteria. Fungal laccases are involved in ecologically important processes such as decomposition of lignocellulose (wood and plant material). In this work, in order to find out the role of fungal laccases upon wood colonisation a...

journal_title：Current genetics

authors： Mäkelä MR,Hildén KS,Hakala TK,Hatakka A,Lundell TK

更新日期：2006-11-01 00:00:00

abstract：:The mating type (MAT) locus of the lentil pathogen, Ascochyta lentis, was cloned and characterized using thermal asymmetric interlaced and inverse PCR with primers designed to the HMG-box of Ascochyta rabiei. A multiplex PCR assay for mating type was developed based on MAT idiomorph and flanking sequences. Primers wer...

journal_title：Current genetics

authors： Chérif M,Chilvers MI,Akamatsu H,Peever TL,Kaiser WJ

更新日期：2006-09-01 00:00:00

abstract：:For a comprehensive evaluation of genes that have their expression modulated during exposure of the mycelia to voriconazole, we performed a large-scale analysis of gene expression in Aspergillus fumigatus using a microarray hybridization approach. By comparing the expression of genes between the reference time and aft...

journal_title：Current genetics

authors： da Silva Ferreira ME,Malavazi I,Savoldi M,Brakhage AA,Goldman MH,Kim HS,Nierman WC,Goldman GH

更新日期：2006-07-01 00:00:00

abstract：:A transformation system utilizing Agrobacterium tumefaciens was developed for targeted gene disruption in Monilinia fructicola, a fungal pathogen that causes brown rot disease of stone fruits. Transformation with a vector containing the neomycin phosphotransferase II (nptII) cassette flanked with 4 kb cutinase gene (M...

journal_title：Current genetics

authors： Lee MH,Bostock RM

更新日期：2006-05-01 00:00:00

abstract：:In order to study pathogenic mechanisms of the plant pathogen Ascochyta rabiei, conditions for efficient transformation using Agrobacterium-mediated transformation were investigated. Hygromycin B resistance (hph) was superior to geneticin resistance (nptII) for selecting transformants, and the hph gene was more effici...

journal_title：Current genetics

authors： White D,Chen W

更新日期：2006-04-01 00:00:00

abstract：:The generation of a comprehensive EST library and the sequencing of its genome set the stage for reverse genetics approaches in Chlamydomonas reinhardtii. However, these also require tools for the specific downregulation of target gene expression. Consequently, a large number of diverse constructs were developed aimed...

journal_title：Current genetics

authors： Schroda M

更新日期：2006-02-01 00:00:00

abstract：:A system for the expression of an ATP binding cassette (ABC) transporter from the soybean pathogen Phytophthora sojae is described. Pdr1, an ABC transporter with homology to the pleiotropic drug resistance (PDR) family of transporters, was cloned by primer walking from a P. sojae genomic library. Reverse transcriptase...

journal_title：Current genetics

authors： Connolly MS,Sakihama Y,Phuntumart V,Jiang Y,Warren F,Mourant L,Morris PF

更新日期：2005-12-01 00:00:00

abstract：:The HOG pathway is an important mitogen-activated protein kinase (MAPK) signal transduction pathway in Saccharomyces cerevisiae that mediates adaptation of cells to hyper-osmotic stress. Activation of this pathway causes rapid but transient, phosphorylation of the MAPK Hog1p. Phosphorylated Hog1p is rapidly transporte...

journal_title：Current genetics

authors： Sharma P,Meena N,Aggarwal M,Mondal AK

更新日期：2005-09-01 00:00:00

abstract：:Mycorrhizal ascomycetes are ecologically and commercially important fungi that have proved impervious to genetic transformation so far. We report here on the successful transient transformation of Tuber borchii, an ectomycorrhizal ascomycete that colonizes a variety of trees and produces highly prized hypogeous fruitb...

journal_title：Current genetics

authors： Grimaldi B,de Raaf MA,Filetici P,Ottonello S,Ballario P

更新日期：2005-07-01 00:00:00

abstract：:The phosphate regulatory mechanism in yeast, known as the PHO pathway, is regulated by inorganic phosphate to control the expression of genes involved in the acquisition of phosphate from the medium. This pathway is also reported to contribute to other nutritional responses and as such it affects several phenotypic ch...

journal_title：Current genetics

authors： Swinnen E,Rosseels J,Winderickx J

更新日期：2005-07-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the HAP transcriptional complex is involved in the fermentation-respiration shift. This complex is composed of four subunits. Three subunits are necessary for DNA-binding, whereas the Hap4p subunit, glucose-repressed, contains the transcriptional activation domain. Hap4p is the key regulat...

journal_title：Current genetics

authors： Sybirna K,Guiard B,Li YF,Bao WG,Bolotin-Fukuhara M,Delahodde A

更新日期：2005-03-01 00:00:00

abstract：:Genetic and molecular analyses of the phenomenon of senescence-i.e., irreversible loss of growth and reproductive potential upon subculturing-in Neurospora intermedia strain M1991-60A, collected from Maddur in southern India, showed the presence of plasmid pMaddur1, which is homologous to the senescence-inducing circu...

journal_title：Current genetics

authors： D'Souza AD,Sultana S,Maheshwari R

更新日期：2005-03-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) repair occurs in all eukaryotic organisms and is essential for the maintenance of mitochondrial function. Evidence from both humans and yeast suggests that mismatch repair is one of the pathways that functions in overall mtDNA stability. In the mitochondria of the yeast Saccharomyces cerevisi...

journal_title：Current genetics

authors： Mookerjee SA,Lyon HD,Sia EA

更新日期：2005-02-01 00:00:00

abstract：:A phylogenetic investigation of the autosporic chlorophycean alga species Selenastrum capricornutum using the small subunit (SSU) rRNA gene revealed the unusual presence of six group IC1 introns. Previous studies showed that numerous green algal taxa contain group IC1 introns. However, whereas some algal species harbo...

journal_title：Current genetics

authors： Booton GC,Floyd GL,Fuerst PA

更新日期：2004-10-01 00:00:00

abstract：:We previously reported the isolation of mutants hypersensitive to the genotoxic agent 4-nitroquinoline-1-oxide, a potent inducer of oxidative stress. One of the mutants was defective in a gene designated yPTPA1, encoding a protein related to the human phosphotyrosyl phosphatase activator hPTPA, which is believed to pl...

journal_title：Current genetics

authors： Douville J,David J,Fortier PK,Ramotar D

更新日期：2004-08-01 00:00:00

abstract：:The Cdc42p GTPase regulates multiple signal transduction pathways through its interactions with downstream effectors. Specific functional domains within Cdc42p are required for guanine-nucleotide binding, interactions with downstream effectors, and membrane localization. However, little is known about how Cdc42p is cl...

journal_title：Current genetics

authors： Richman TJ,Toenjes KA,Morales SE,Cole KC,Wasserman BT,Taylor CM,Koster JA,Whelihan MF,Johnson DI

更新日期：2004-06-01 00:00:00

abstract：:Fission yeast is a simple eukaryotic model organism in which many aspects of cell cycle control can be explored. We examined by homologous recombination whether the human CDC25A phosphatase could substitute for the function of the fission yeast Cdc25. We first show: (a). that CDC25A efficiently replaces the endogenous...

journal_title：Current genetics

authors： Mondesert O,Lemaire M,Brezak MC,Galera-Contour MO,Prevost G,Ducommun B,Bugler B

更新日期：2004-05-01 00:00:00

abstract：:In the fungus Fusarium sporotrichioides, biosynthesis of trichothecene mycotoxins requires at least three genetic loci: a core 12-gene cluster, a smaller two-gene cluster, and a single-gene locus. Here, we describe the Tri15 gene, which represents a fourth locus involved in trichothecene biosynthesis. Tri15 is predict...

journal_title：Current genetics

authors： Alexander NJ,McCormick SP,Larson TM,Jurgenson JE

更新日期：2004-03-01 00:00:00

abstract：:The mating-type ( MAT) genes from Rhynchosporium secalis were isolated using PCR-based methods. Characterisation of the MAT idiomorphs suggests that R. secalis is closely related to the discomycetes Pyrenopeziza brassicae and Tapesia yallundae in terms of sequence and MAT locus gene composition. The MAT1-2 idiomorph c...

journal_title：Current genetics

authors： Foster SJ,Fitt BD

更新日期：2003-12-01 00:00:00

abstract：:As part of developing an ex planta model system for the study of maize plastid and mitochondrial gene expression, a series of established Black Mexican Sweet (BMS) suspension cell lines was characterized. Although the initial assumption was that their organelle biochemistry would be similar enough to normal in planta ...

journal_title：Current genetics

authors： Cahoon AB,Cunningham KA,Bollenbach TJ,Stern DB

更新日期：2003-11-01 00:00:00

abstract：:Mitochondrial genomes in organisms from diverse phylogenetic groups vary in both size and molecular form. Although the types of mitochondrial genome appear very dissimilar, several lines of evidence argue that they do not differ radically. This would imply that interconversion between different types of mitochondrial ...

journal_title：Current genetics

authors： Nosek J,Tomáska L

更新日期：2003-11-01 00:00:00

abstract：:Chaperonin 60 (Cpn60) is a well-established marker protein for eukaryotic mitochondria and plastids. In order to determine whether the small double-membrane-bounded organelle posterior to the nucleus in the apicomplexan Cryptosporidium parvum is a mitochondrion, the Cpn60 gene of C. parvum sporozoites ( CpCpn60) was a...

journal_title：Current genetics

authors： Riordan CE,Ault JG,Langreth SG,Keithly JS

更新日期：2003-11-01 00:00:00

abstract：:International efforts are underway that aim at determining the complete genome sequence of the social amoeba Dictyostelium discoideum. As strategy, a whole chromosome shotgun (WCS) approach was chosen and each of the six Dictyostelium chromosomes was assigned to project partners. The project is well advanced, chromoso...

journal_title：Current genetics

authors： Eichinger L

更新日期：2003-11-01 00:00:00

abstract：:The nucleotide sequences of regions containing the mating-type locus of the plant-pathogenic ascomycete Leptosphaeria maculans are described. The MAT1-1 gene is 1,368 bp, encoding a predicted protein of 441 amino acids, with a 45-bp intron. The MAT1-2 gene is 1,246 bp, encoding a predicted protein of 397 amino acids, ...

journal_title：Current genetics

authors： Cozijnsen AJ,Howlett BJ

更新日期：2003-08-01 00:00:00

abstract：:A double-stranded (ds)RNA, designated as M2, is associated with hypovirulence, conversion of the quinic acid pathway from inducible to constitutive and downregulation of the shikimic acid pathway in the Rhizoctonia solani culture Rhs 1A1. In this study, we report that in the virulent, M2-lacking isolate Rhs 1AP, which...

journal_title：Current genetics

authors： Liu C,Lakshman DK,Tavantzis SM

更新日期：2003-05-01 00:00:00

abstract：:O-Mannosylation is suggested to be essential for protein secretion in Trichoderma reesei. In protein O-glycosylation, the first mannosyl residue is transferred to a serine or threonine hydroxyl group of the protein from dolichyl phosphate mannose by protein O-mannosyltransferase. In Saccharomyces cerevisiae, seven PMT...

journal_title：Current genetics

authors： Zakrzewska A,Migdalski A,Saloheimo M,Penttila ME,Palamarczyk G,Kruszewska JS

更新日期：2003-04-01 00:00:00

abstract：:Mitotic homologous recombination (HR) is an important mechanism for the repair of double-strand breakS and errors occurring during DNA replication. It is likely that the recombinational repair of DNA lesions occurs preferentially by sister chromatid exchanges that have no genetic consequences. However, most geneticall...

journal_title：Current genetics

authors： Prado F,Cortés-Ledesma F,Huertas P,Aguilera A

更新日期：2003-01-01 00:00:00

abstract：:In order to analyse gene expression during fruit body development of the ectomychorrizal fungus Tuber borchii Vittad., a modified differential display procedure was set up. The procedure used is easier and faster than the traditional one and generates reproducible cDNA banding patterns that can be resolved on a standa...

journal_title：Current genetics

authors： Zeppa S,Guidi C,Zambonelli A,Potenza L,Vallorani L,Pierleoni R,Sacconi C,Stocchi V

更新日期：2002-12-01 00:00:00

abstract：:The presence of the endochitinase CHIT36 from Trichoderma harzianum TM was assessed in several antagonistic Trichoderma strains belonging to different molecular taxonomic groups. CHIT37 from T. harzianum CECT 2413 was sequenced and found to display 89% homology with CHIT36 at the amino acid level. Northern analysis sh...

journal_title：Current genetics

authors： Viterbo A,Montero M,Ramot O,Friesem D,Monte E,Llobell A,Chet I

更新日期：2002-11-01 00:00:00

abstract：:As yeast colonies ceased growth, cells at the edge of these colonies transited from the cell division cycle into meiosis at high efficiency. This transition occurred remarkably synchronously and only at late stages of colony maturation. The transition occurred on medium containing acetate or low concentrations of gluc...

journal_title：Current genetics

authors： Purnapatre K,Honigberg SM

更新日期：2002-10-01 00:00:00